Marker-exchange mutagenesis and complementation strategies for the Gram-negative bacteria Xanthomonas oryzae pv. oryzae.
نویسندگان
چکیده
This chapter describes methods for targeted knockouts using marker exchange mutagenesis and complementation of the Gram-negative bacteria Xanthomonas oryzae pv. oryzae. We have used these methods to demonstrate that type I secretion and modification systems are involved in avrXa21 activity of X. oryzae pv. oryzae.
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ورودعنوان ژورنال:
- Methods in molecular biology
دوره 354 شماره
صفحات -
تاریخ انتشار 2007